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    Area of Science:

    • Biomedical Optics
    • Microscopy
    • Biophotonics

    Background:

    • Full-field optical coherence microscopy (FF-OCM) offers label-free imaging.
    • Achieving high isotropic spatial resolution is crucial for visualizing sub-cellular structures.

    Purpose of the Study:

    • To develop and characterize an ultrahigh-resolution FF-OCM system.
    • To investigate the axial response and optimize resolution for biological tissue imaging.

    Main Methods:

    • Theoretical analysis of FF-OCM axial response, considering optical dispersion.
    • Optimization of lateral resolution using water-immersion objectives (NA=1.2).
    • Imaging of animal and human skin tissues at 700 nm center wavelength.

    Main Results:

    • Achieved isotropic spatial resolution of 0.5 μm in water.
    • Demonstrated visualization of ultra-fine, in-depth structures at the sub-cellular level.
    • Successful application to diverse skin tissue samples.

    Conclusions:

    • The developed FF-OCM system provides unprecedented sub-cellular resolution for biological imaging.
    • This technology advances the in-vivo and ex-vivo study of tissue microstructure.
    • FF-OCM is a powerful tool for biomedical research and diagnostics.