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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

Updated: Mar 29, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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SPIM-fluid: open source light-sheet based platform for high-throughput imaging.

Emilio J Gualda1, Hugo Pereira2, Tiago Vale1

  • 1Advance Imaging Lab, Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6,Oeiras 2780-156, Portugal.

Biomedical Optics Express
|November 25, 2015
PubMed
Summary
This summary is machine-generated.

We developed an open-source system for automated 3D imaging using light sheet fluorescence microscopy. This approach enables high-throughput screening of drugs on model organisms and cell cultures with minimal photodamage.

Keywords:
(110.6880) Three-dimensional image acquisition(180.2520) Fluorescence microscopy

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Area of Science:

  • Biomedical Engineering
  • Microscopy Technology
  • Drug Discovery

Background:

  • Light sheet fluorescence microscopy (LSFM) offers high-quality 3D imaging with low photodamage and fast acquisition.
  • LSFM is increasingly vital for biological research, particularly in developmental biology and high-throughput screening.

Purpose of the Study:

  • To present an open-source, unified implementation for operating LSFMs.
  • To enable automated, high-throughput 3D imaging for drug screening applications.

Main Methods:

  • Developed a system integrating Arduino and Micro-Manager software.
  • Implemented automated 3D imaging capabilities for LSFM.
  • Validated the system on three-dimensional cell cultures and zebrafish embryos.

Main Results:

  • Achieved automated, high-throughput 3D imaging using LSFM.
  • Demonstrated low photodamage and fast acquisition rates.
  • Successfully applied the system to model organisms and cell cultures for screening.

Conclusions:

  • The presented open-source LSFM system facilitates automated, high-throughput 3D imaging.
  • This implementation is suitable for large-scale drug screening and biological research.
  • The unified platform enhances accessibility and efficiency in LSFM applications.