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Updated: Mar 28, 2026

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
Published on: April 21, 2023
Photo-lysine captures proteins that bind lysine post-translational modifications
Tangpo Yang1, Xiao-Meng Li1, Xiucong Bao1
1Department of Chemistry, University of Hong Kong, Pokfulam Road, Hong Kong, China.
Abstract:
Post-translational modifications (PTMs) have key roles in regulating protein-protein interactions in living cells. However, it remains a challenge to identify these PTM-mediated interactions. Here we develop a new lysine-based photo-reactive amino acid, termed photo-lysine. We demonstrate that photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine PTMs, including 'readers' and 'erasers' of histone modifications.
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