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Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
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Selective diffusion spectroscopy using excitation sculpting.

Peter W A Howe1

  • 1Product Safety, Syngenta Ltd, Jealott's Hill Research Centre, Bracknell, RG42 6EY, UK.

Magnetic Resonance in Chemistry : MRC
|December 30, 2015
PubMed
Summary
This summary is machine-generated.

Excitation sculpting in Nuclear Magnetic Resonance (NMR) spectroscopy improves diffusion coefficient measurements by suppressing intense signals. This technique allows for accurate diffusion measurements of minor components, even in complex samples like polymeric solvents.

Keywords:
1HNMRconvection compensationdiffusion spectroscopyexcitation sculptingselective excitationspin-echo

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Diffusion spectroscopy NMR is a rapid method for determining diffusion coefficients.
  • Fitting resonance intensities to the Stejskal-Tanner equation is sensitive to experimental artifacts, especially intense solvent signals.
  • Artifacts from intense resonances can distort diffusion coefficient measurements of minor solutes.

Purpose of the Study:

  • To demonstrate the direct application of excitation sculpting pulse sequences for DOSY (Diffusion Ordered Spectroscopy) NMR.
  • To overcome challenges in diffusion spectroscopy caused by intense solvent resonances.
  • To enable reproducible diffusion coefficient measurements in complex sample matrices.

Main Methods:

  • Utilized a conventional excitation sculpting pulse sequence with minimal modifications for DOSY spectroscopy.
  • Applied selective excitation to suppress intense solvent resonances.
  • Investigated the robustness of the method against convection.

Main Results:

  • The modified excitation sculpting sequence effectively suppressed intense resonances in a solvent with five separate signals.
  • Reproducible diffusion coefficients were obtained for minor solutes.
  • The method demonstrated sensitivity and robustness to convection.

Conclusions:

  • Excitation sculpting is a viable and effective method for enhancing DOSY spectroscopy.
  • This technique broadens the applicability of diffusion spectroscopy to samples with multiple intense resonances, such as polymeric solvents.
  • The method offers a robust solution for accurate diffusion measurements in challenging NMR samples.