Inhibiting cyprinid herpesvirus-3 replication with CRISPR/Cas9
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Updated: Mar 28, 2026
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
Published on: January 7, 2019
Yicheng Zhao1,2, Tiedong Wang2, Ze Yu1
1College of Life Sciences, Northeast Forestry University, Harbin, China.
Objectives:
The potential of CRISPR/Cas9 gene editing to repress CyHV-3 was tested in vitro.
Results:
By targeting two basic target genes necessary for the early transcription of CyHV-3, we show that virus transcription and particle release were significantly decreased by CRISPR/Cas9, as measured by quantitative real-time PCR and virus titration experiments, respectively.
Conclusions:
(A) The effectiveness is confirmed of the CRISPR/Cas9 system at repressing exogenous genes, including large viral genomic DNA, by introducing site-specific mutations in vitro. (B) The CyHV-3 virus replicates poorly in Cas9-positive cells. (C) The inhibition of thymidine kinase alone cannot block viral particle release.
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