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Updated: Mar 27, 2026

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
Published on: December 8, 2010
Tissue Discs: A 3D Model for Assessing Modulation of Tissue Oxygenation
M J Gandolfo1, A H Kyle1, A I Minchinton2
1Department of Integrative Oncology, BC Cancer Research Centre, Vancouver, BC, Canada.
Abstract:
The presence of hypoxia in solid tumours is correlated with poor treatment outcome. We have developed a 3-D tissue engineered construct to quantitatively monitor oxygen penetration through tumour tissue using the exogenous 2-nitroimidazole bioreductive probe pimonidazole and phosphorescence quenching technologies. Using this in vitro model we were able to examine the influence of the biguanides metformin and phenformin, antimycin A and KCN, on the distribution and kinetics of oxygen delivery as prototypes of modulators of oxygen metabolism.
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