Related Experiment Video
Updated: Mar 25, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
Published on: October 9, 2014
Fractional Diffusion, Low Exponent Lévy Stable Laws, and 'Slow Motion' Denoising of Helium Ion Microscope Nanoscale
Alfred S Carasso1, András E Vladár1
1National Institute of Standards and Technology, Gaithersburg, MD 20899.
Abstract:
Helium ion microscopes (HIM) are capable of acquiring images with better than 1 nm resolution, and HIM images are particularly rich in morphological surface details. However, such images are generally quite noisy. A major challenge is to denoise these images while preserving delicate surface information. This paper presents a powerful slow motion denoising technique, based on solving linear fractional diffusion equations forward in time. The method is easily implemented computationally, using fast Fourier transform (FFT) algorithms. When applied to actual HIM images, the method is found to reproduce the essential surface morphology of the sample with high fidelity. In contrast, such highly sophisticated methodologies as Curvelet Transform denoising, and Total Variation denoising using split Bregman iterations, are found to eliminate vital fine scale information, along with the noise. Image Lipschitz exponents are a useful image metrology tool for quantifying the fine structure content in an image. In this paper, this tool is applied to rank order the above three distinct denoising approaches, in terms of their texture preserving properties. In several denoising experiments on actual HIM images, it was found that fractional diffusion smoothing performed noticeably better than split Bregman TV, which in turn, performed slightly better than Curvelet denoising.
More Related Videos
05:56Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Published on: November 12, 2020
11:03An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids
Published on: December 4, 2017
Related Concept Videos
Behavior of Gas Molecules: Molecular Diffusion, Mean Free Path, and Effusion
Extraction: Partition and Distribution Coefficients
For extracting a solute from an aqueous phase into an...
Physiological Pharmacokinetic Models: Blood Flow-Limited Versus Diffusion-Limited Models
First Law: Particles in One-dimensional Equilibrium
First Law: Particles in Two-dimensional Equilibrium
Newton's first law tells us about...
The Integrated Rate Law: The Dependence of Concentration on Time