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    Area of Science:

    • Optical microscopy
    • Holography
    • Imaging systems

    Background:

    • Off-axis holographic microscopy offers high-resolution imaging but is limited by a narrow field of view.
    • Multiplexing techniques can increase the field of view in imaging systems.

    Purpose of the Study:

    • To develop a novel multiplexed off-axis holographic microscope with an expanded field of view.
    • To demonstrate the system's capability to image multiple areas simultaneously without compromising resolution.

    Main Methods:

    • Utilized a 1D holographic grating to generate multiple object waves.
    • Interfered four object waves with a reference wave on an imaging sensor to create a multiplexed hologram.
    • Acquired amplitude and phase profiles from four imaging areas using a single exposure.

    Main Results:

    • Successfully retrieved amplitude and phase information from four distinct imaging areas.
    • Demonstrated a significant increase in the field of view compared to conventional off-axis holographic microscopes.
    • Verified the system's performance using a USAF 1951 resolution target and a paramecia slide, showing no loss in resolution.

    Conclusions:

    • The novel multiplexed off-axis holographic microscope effectively expands the field of view.
    • The system provides high-resolution imaging of multiple areas simultaneously.
    • This technique offers a promising advancement for holographic microscopy applications.