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Updated: Mar 24, 2026
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
Published on: January 22, 2020
[Tubacin promotes Foxp3 expression and suppressive function of mouse CD4+;CD25+; regulatory T cells]
Shuguang Zhao1, Ziying Chen2, Ding Yu1
1Department of Cardiac Surgery, Second Affiliated Hospital, Hebei Medical University, Shijiazhuang 050000, China.
Objective:
To observe the changes of fork head transcription factor (Foxp3) expressions and suppressive functions of mouse regulatory T cells (Tregs) after stimulated by tubacin (histone deacetylase 6 inhibitor) in vitro.
Methods:
CD4(+) CD25(+) T and CD4(+) CD25(-) T cells were gained from C57BL/6J mouse spleen lymphocytes by double positive magnetic bead sorting method. Natural Tregs (nTregs) and transforming growth factor β1 (TGF-β1)-induced Tregs (iTregs) were stimulated by tubacin in vitro. Foxp3 expression of each group was identified by reverse transcription PCR, and immunosuppressive activities of each group were tested by the mixed lymphocyte culture (MLC).
Results:
Foxp3 expression of nTregs and iTregs were significantly enhanced after stimulated with tubacin. The tubacin induced CD4(+) CD25(+) Foxp3(high) Tregs significantly inhibited syngeneic CD4(+) CD25(-) T cell activation.
Conclusion:
Tubacin can upregulate Foxp3 expression of CD4(+) CD25(+) Tregs and enhances their cellular immunosuppressive capability.
Insights
Tubacin, a histone deacetylase 6 inhibitor, boosts regulatory T cell (Treg) function. This study shows tubacin increases Foxp3 expression and enhances the immunosuppressive capabilities of mouse Tregs in vitro.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Regulatory T cells (Tregs) are crucial for immune homeostasis.
- Foxp3 is a key transcription factor defining Treg lineage and function.
- Histone deacetylase 6 (HDAC6) inhibitors are being explored for immunomodulatory effects.
Purpose of the Study:
- To investigate the effect of tubacin, an HDAC6 inhibitor, on Foxp3 expression in mouse Tregs.
- To assess the impact of tubacin on the suppressive functions of mouse Tregs in vitro.
Main Methods:
- Isolation of CD4(+) CD25(+) and CD4(+) CD25(-) T cells from C57BL/6J mice.
- In vitro stimulation of natural Tregs (nTregs) and TGF-β1-induced Tregs (iTregs) with tubacin.
- Quantification of Foxp3 expression using reverse transcription PCR.
- Evaluation of immunosuppressive activity via mixed lymphocyte culture (MLC).
Main Results:
- Tubacin significantly upregulated Foxp3 expression in both nTregs and iTregs.
- Tubacin-induced CD4(+) CD25(+) Foxp3(high) Tregs demonstrated enhanced inhibition of syngeneic CD4(+) CD25(-) T cell activation.
Conclusions:
- Tubacin effectively upregulates Foxp3 expression in CD4(+) CD25(+) Tregs.
- Tubacin enhances the immunosuppressive capacity of Tregs, suggesting potential therapeutic applications.
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