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Neutrophil activation during hemodialysis
M Haag-Weber1, P Schollmeyer, W H Hörl
1Department of Medicine, University of Freiburg, FRG.
Advances in Experimental Medicine and Biology
|January 1, 1989
Summary
Uremic patients exhibit enhanced polymorphonuclear cell (PMN) oxidative metabolism, which hemodialysis (HD) partially normalizes. Hemodialysis impacts PMN function, affecting respiratory burst and carbohydrate metabolism, with dialyzer material influencing outcomes.
Area of Science:
- Nephrology
- Immunology
- Biochemistry
Background:
- Uremic patients exhibit altered polymorphonuclear cell (PMN) oxidative metabolism.
- Serum factors in uremia enhance PMN oxidative metabolism, and these factors are removed by hemodialysis (HD).
- PMN respiratory burst activity is diminished in uremic patients.
Purpose of the Study:
- To investigate the effects of hemodialysis on PMN oxidative metabolism and function in uremic patients.
- To explore the role of serum factors and dialyzer membranes in modulating PMN activity during HD.
Main Methods:
- Assessment of PMN respiratory burst activity (hydrogen peroxide production) in stimulated and unstimulated states.
- Measurement of chemiluminescence (CL) correlating with complement activation.
- Analysis of PMN carbohydrate metabolism (glycogenolysis) during HD with different dialyzer materials (polysulfone, PMMA).
- Evaluation of degranulation and the effect of calcium channel blockers.
Main Results:
- Hemodialysis normalized stimulated hydrogen peroxide production while decreasing unstimulated production in uremic PMNs.
- Chemiluminescence during HD showed correlation with complement activation, though some dialyzers caused minimal anaphylatoxin formation.
- Polysulfone dialyzers improved PMN carbohydrate metabolism, whereas PMMA activated glycogenolysis.
- PMN degranulation occurred independently of complement activation, and calcium channel blockers reduced PMN activation.
Conclusions:
- Hemodialysis partially restores PMN oxidative metabolism in uremic patients.
- Dialyzer membrane composition influences PMN carbohydrate metabolism and activation.
- Alterations in PMN function during HD may contribute to phagocytosis disturbances.