CHANGES OF LIPOPEROXIDATION AND ANTIOXIDATIVE ENZYMES DURING CRUSH-SYNDROME MODELLING

Crush-syndrome (CS) is characterized by numerous pathological deviations due to the soft tissue damage and their further reperfusion. The aim of the study was to investigate pro- and antioxidative processes during different regimens of crush syndrome. The experiment was carried out on randomly selected 200-250gr. mass 50 laboratory rats using crush syndrome modeling classical method. Investigations were conducted at various stages of compression and decompression period. Activity of antioxidant enzymes - total ceruloplasmin, oxidized ceruloplasmin was determined in blood serum. LOO. and free oxygen species were as well determined with the use of relevant methods. According to our findings we can conclude that: - Lipoperoxidation intensity increases in compliance with crush syndrome duration; - Short-term (3-hour) compression causes enhancement of lipoperoxidation however, in further 1 hour decompression there is revealed a trend toward normalization of processes. Lipoperoxides and free oxygen species content decreases and the antioxidant enzymes activity is almost restored; - Long lasting compression (6 hours) leads to severe disorders in the body (total ceruloplasmin impaired production and after 6 hours from decompression antioxidant enzymes inactivation).