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In Vivo Depletion of T Lymphocytes.
1National Institutes of Health, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
Current Protocols in Immunology
|April 3, 2016
Summary
This study details a method for depleting T lymphocytes in mice using monoclonal antibodies to investigate their roles in immune responses. This technique allows researchers to study specific T cell functions in vivo.
Area of Science:
- Immunology
- Cell Biology
Background:
- Studying T cell populations in vivo is crucial for understanding immune responses.
- Monoclonal antibodies (mAbs) offer a targeted approach for cell depletion.
Purpose of the Study:
- To provide a protocol for in vivo T lymphocyte depletion using monoclonal antibodies in adult mice.
- To enable the study of specific T cell subset functions during immune responses.
Main Methods:
- Administration of monoclonal antibodies (mAbs) to wild-type adult mice.
- Verification of cell depletion via flow cytometry analysis of lymph node and spleen cells.
- Utilizing depleted mice to investigate the impact of T cell subsets on in vivo immune responses.
Main Results:
- Established protocol for effective in vivo T lymphocyte depletion.
- Demonstrated verification of T cell subset depletion using flow cytometry.
- Enabled further research into the functional roles of specific T cell populations.
Conclusions:
- In vivo T cell depletion using mAbs is a viable method for immunological research.
- This protocol facilitates the investigation of T cell subset functions in various immune contexts.
- The depletion can be sustained or reversed, offering experimental flexibility.
