Related Experiment Video
Updated: Mar 22, 2026
Intraoperative Assessment of Resection Margins in Oral Cavity Cancer: This is the Way
Published on: May 10, 2021
Rapid Lump Examination as a New Aid to Speedup Mohs Micrographic Surgery: A Pilot Study
Marleen Veenstra1, Judith Ostertag, Marc Verhaegh
1*Mohs Klinieken Eindhoven, Eindhoven, the Netherlands; †Universitäts-Hautklinik Tübingen, Tübingen, Germany; ‡Praxisklinik Tübingen-Haut und Venen, Dr. A. Bern und Prof. Dr. M. Möhrle, Tübingen, Germany.
Background:
Although most cases of basal cell carcinoma (BCC) are curable, facial and recurrent BCCs require time consuming and labor-intensive Mohs micrographic surgery (MMS) to ensure complete removal with optimal cosmetic results. To handle the skin cancer epidemic, there is an urgent need to speedup MMS. Recently, rapid lump examination (RLE) has been shown to enable quick histologic examination, but its usefulness within MMS is unknown.
Objective:
To evaluate the accuracy of RLE for the detection of residual BCC during MMS.
Materials And Methods:
Twenty-two BCCs of various subtypes were evaluated by means of RLE and standard fresh frozen section (FFS) examination. For RLE, the freshly excised tissue was stained with toluidine blue and imaged with a stereomicroscope before being subdivided into sections suitable for processing into FFS. Fresh frozen sections were stained with hemotoxylin and eosin and evaluated with light microscopy.
Results:
Using FFS examination as the gold standard for the detection of residual BCC, RLE had a sensitivity, specificity, positive predictive value, and negative predictive value of 83.8%, 94.0%, 83.8%, and 94.0%, respectively.
Conclusion:
As RLE enables quick 3-dimensional visualization of surgical margins for the detection of residual BCC, it holds the potential to speedup histologic examination during MMS.
