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Updated: Mar 22, 2026
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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
Published on: January 19, 2017
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Replication timing and transcriptional control: beyond cause and effect-part III.
Juan Carlos Rivera-Mulia1, David M Gilbert2
1Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA.
Current Opinion in Cell Biology
|April 27, 2016
Summary
DNA replication timing (RT) follows a program, organized into replication domains (RDs) that also function as topologically associating domains (TADs). These domains show complex, context-dependent relationships with gene regulation and chromatin organization during development.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- DNA replication is crucial for genetic information transfer and is linked to chromosome structure.
- Genome duplication follows a specific temporal order, the replication-timing (RT) program.
- Replication domains (RDs) regulate this program during cell cycle and development, corresponding to topologically associating domains (TADs).
Purpose of the Study:
- To investigate the complex relationships between replication timing, chromatin organization, and gene regulation.
- To understand how replication domains (RDs) and topologically associating domains (TADs) are regulated during development.
- To challenge existing correlations between RT and chromatin organization/gene regulation.
Main Methods:
- Utilizing advanced experimental tools to study genome duplication.
- Analyzing the spatio-temporal compartmentalization of RDs/TADs within the nucleus.
- Investigating context-dependent relationships in RT during development.
Main Results:
- Experimental tools reveal complex, context-dependent relationships in RT.
- Long-recognized correlations between RT and chromatin organization/gene regulation are challenged.
- Replication domains/TADs that switch RT during development exhibit unique regulatory patterns.
Conclusions:
- Replication timing is intricately linked to chromosome structure and gene regulation.
- The regulation of RDs/TADs is complex and context-dependent, especially during development.
- Further research is needed to fully elucidate the causality and mechanisms governing RT programs.
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