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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
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In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules
Kyung-Ae Yang1, Renjun Pei2, Milan N Stojanovic3
1Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, United States.
Methods (San Diego, Calif.)
|May 8, 2016
Abstract:
We recently optimized a procedure that directly yields aptameric sensors for small molecules in so-called structure-switching format. The protocol has a high success rate, short time, and is sufficiently simple to be readily implemented in a non-specialist laboratory. We provide a stepwise guide to this selection protocol.
