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Morphological Changes in Mesenteric Lymph Nodes and Lymphocyte Subpopulation Composition in Experimental Ulcerative
E A Postovalova1,2, D N Khochansky3, N A Zolotova4,3
1Department of Cellular Biology and Histology, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia. e.a.postovalova@gmail.com.
Bulletin of Experimental Biology and Medicine
|May 12, 2016
Summary
Investigating ulcerative colitis in mice revealed significant changes in mesenteric lymph nodes. Acute phases showed decreased B cells and increased cytotoxic T cells, while chronic phases exhibited elevated B cells and T helper cells, indicating immune response shifts.
Area of Science:
- Immunology
- Gastroenterology
- Pathology
Background:
- Ulcerative colitis (UC) is a chronic inflammatory bowel disease.
- Mesenteric lymph nodes (MLNs) play a crucial role in gut immunity.
- Understanding MLN changes in UC is vital for therapeutic development.
Purpose of the Study:
- To investigate morphological and lymphocyte subpopulation changes in MLNs during experimental UC.
- To differentiate immune responses in acute versus chronic UC models.
Main Methods:
- Induction of acute and chronic ulcerative colitis in male C57Bl/6 mice using sodium dextran sulfate.
- Analysis of MLN morphology, including follicular hyperplasia and sinus reactions.
- Flow cytometry to quantify lymphocyte subpopulations (CD19+ B cells, CD3+CD8+ cytotoxic T cells, CD4+CD25+FOXP3+ regulatory T cells, CD4+ T helper cells).
Main Results:
- Both acute and chronic UC induced reactive changes in MLNs.
- Acute UC: Decreased CD19+ B cells, increased CD3+CD8+ cytotoxic T cells, suggesting Th1 activation.
- Chronic UC: Increased CD19+ B cells and CD3+CD4+ T helper cells, indicative of adaptive immunity and inflammation chronicity.
Conclusions:
- MLN immune dynamics differ significantly between acute and chronic experimental UC.
- Lymphocyte migration from thymus and colon contributes to MLN changes.
- Findings highlight distinct immune profiles in UC progression, offering targets for intervention.
