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Updated: Mar 21, 2026

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants
Published on: September 12, 2018
Laboratory course on Streptomyces genetics and secondary metabolism
Vilja Siitonen1, Kaj Räty2, Mikko Metsä-Ketelä2
1Department of Biochemistry, University of Turku, Turku, FIN-20014, Finland. vilja.siitonen@gmail.com.
Abstract:
The "Streptomyces genetics and secondary metabolism" laboratory course gives an introduction to the versatile soil dwelling Gram-positive bacteria Streptomyces and their secondary metabolism. The course combines genetic modification of Streptomyces; growing of the strain and protoplast preparation, plasmid isolation by alkaline lysis and phenol precipitation, digestions, and ligations prior to protoplast transformation, as well as investigating the secondary metabolites produced by the strains. Thus, the course is a combination of microbiology, molecular biology, and chemistry. After the course the students should understand the relationship between genes, proteins, and the produced metabolites. © 2016 by The International Union of Biochemistry and Molecular Biology, 44(5):492-499, 2016.
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