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Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

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CatacLysMic specificity when targeting myeloid cells?

Thomas Blank¹, Marco Prinz^1,2

¹Institute of Neuropathology, Freiburg University Medical Centre, Freiburg, Germany.

European Journal of Immunology

|May 21, 2016

Summary

This summary is machine-generated.

LysM-Cre mice, used for targeting myeloid cells, show unexpected reporter expression in brain neurons, not just microglia. This raises concerns about the specificity of LysM-Cre for targeting myeloid cells in research.

Keywords:

Brain LysM-Cre Lyz2 Microglia Neuroinflammation Neurons

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Area of Science:

Immunology
Neuroscience
Genetics

Background:

Lysozyme M (LysM) is a marker for myeloid cells in mice.
LysM-Cre mouse lines are used to target genes in myeloid cells.
Previous studies assumed LysM-Cre specifically targets myeloid cells.

Purpose of the Study:

To verify LysM-driven recombination using reporter mouse lines.
To assess the specificity of LysM-Cre for targeting myeloid cells, particularly microglia.
To evaluate the implications of LysM-Cre usage in past research.

Main Methods:

Generation of a Cre-recombinase (Cre) expressing mouse line by inserting Cre into the LysM gene.
Utilizing tdTomato and YFP reporter mouse lines to track recombination.
Analyzing reporter protein expression in the central nervous system (CNS) and peripheral myeloid cells.

Main Results:

Major tdTomato reporter protein expression was observed in brain neurons.
Only a small percentage of gene recombination occurred in brain myeloid cells (microglia).
LysM-driven recombination is not specific to myeloid cells as previously assumed.

Conclusions:

LysM-Cre mice exhibit significant recombination in non-myeloid cells, including neurons.
Concerns exist regarding the efficiency and specificity of targeting microglia and peripheral myeloid cells using LysM-Cre.
Past studies on CNS diseases using LysM-Cre may require re-evaluation due to potential off-target effects.