Microarray data analyses of yeast RNA Pol I subunit RPA12 deletion strain
Kamlesh Kumar Yadav1, Ram Rajasekharan1
1Lipidomic Center, Department of Lipid Science, CSIR-Central Food Technological Research Institute (CFTRI), Mysore 570020, Karnataka, India.
Genomics Data
|May 26, 2016
Summary
The ribosomal RNA (rRNA) synthesis protein RPA12 regulates cellular metabolism. Deleting RPA12 causes uncontrolled metabolite synthesis, suggesting it
Area of Science:
- Cellular Biology
- Molecular Biology
- Metabolic Regulation
Background:
- Ribosomal RNA (rRNA) biosynthesis is a major energy-consuming process in cells.
- RPA12 is a small, abundant protein involved in rRNA synthesis termination.
- The broader physiological role of RPA12 in cellular metabolism remains largely unknown.
Purpose of the Study:
- To investigate the detailed physiological role of RPA12 in Saccharomyces cerevisiae.
- To understand RPA12's function beyond its known role in rRNA synthesis termination.
Main Methods:
- Gene expression analysis using microarrays on an rpa12 deletion strain (rpa12 ∆).
- Data deposited in Gene Expression Omnibus (GEO) under accession number GSE68731.
Main Results:
- Microarray analysis revealed significantly high expression of major cellular metabolism genes in rpa12 ∆ cells.
- Genes involved in amino acid biosynthesis, nonpolar lipid biosynthesis, and glucose metabolism were highly expressed.
- rpa12 ∆ cells exhibited uncontrolled synthesis of various cell metabolites.
Conclusions:
- RPA12 plays a critical role in regulating overall cellular metabolism.
- RPA12 may function as a master regulator controlling the synthesis of diverse cell metabolites.
