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Updated: Mar 20, 2026
Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
Published on: September 17, 2021
[DETECTION OF DEGREE OF MICROECOLOGICAL DISTURBANCE OF INTESTINE BASED ON CALCULATION OF LOCAL ANTIOXIDANT INDEX]
Aim:
Evaluation of dysbiotic condition of intestine based on determination of local antioxidant index (LAI).
Materials And Methods:
155 patients with bacteriologically confirmed intestine dysbacteriosis were examined: 20 individuals with normobiocenosis of the intestine served as control. Feces dilution 10⁻² was used for biochemical study. Catalase, superoxide-dismutase activity and level of malonic dialdehyde was evaluated photometrically by the value of optical density of the samples. Microbial landscape of feces was studied by a bacteriologic method with parallel determination in samples of coprofiltrates of activity of the indicated enzymes and content of malonic dialdehyde. Evaluation of disturbances of microecology of the intestine was carried out by a biochemical method by calculating local antioxidant index in juxtaposition with microbial map data.
Results:
LAI parameter was significantly higher than 20 in coprofiltrates of people with intestine normobiocenosis. In patients with detected intestine dysbacteriosis, depending on its degree, LAI parameter, changes were registered in the range from negative to 20. Dysbacteriosis in a part of patients had a prolonged character, LAI was lower than 14, that reflected the presence of a persistent inflammation. A tendency of normalization of microbial ecology and growth of LAI was observed during administration of a probiotic preparation.
Conclusion:
A screening criteria is proposed, that differentiates the degree of severity of intestine dysbacteriosis based on calculation of local antioxidant index.
