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Updated: Mar 20, 2026

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
Published on: August 23, 2024
Precise and rapid isotopomic analysis by (1)H-(13)C 2D NMR: Application to triacylglycerol matrices
Noelle Merchak1, Virginie Silvestre2, Laetitia Rouger2
1EBSI team, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling (CEISAM), University of Nantes-CNRS UMR 6230, 2 rue de la Houssinière, BP 92208, F-44322 Nantes cedex 3, France; Research Unit: Technologies et Valorisation Agroalimentaire (TVA), Laboratory of Metrology and Isotopic Fractionation, Faculty of Science, Saint-Joseph University, P.O. Box 11-514 Riad el Solh, Beirut 1107 2050, Lebanon.
Abstract:
An optimized HSQC sequence was tested and applied to triacylglycerol matrices to determine their isotopic and metabolomic profiles. Spectral aliasing and non-uniform sampling approaches were used to decrease the experimental time and to improve the resolution, respectively. An excellent long-term repeatability of signal integrals was achieved enabling to perform isotopic measurements. Thirty-two commercial vegetable oils were analyzed by this methodology. The results show that this method can be used to classify oil samples according to their geographical and botanical origins.
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