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Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
Published on: September 28, 2019
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Design Principles for Engineering of Tissues from Human Pluripotent Stem Cells
Oriane B Matthys1, Tracy A Hookway2, Todd C McDevitt3
1The Gladstone Institute of Cardiovascular Disease, San Francisco, CA; Graduate Program in Bioengineering, University of California Berkeley and University of California San Francisco, Berkeley/San Francisco, CA.
Current Stem Cell Reports
|June 23, 2016
Summary
Human pluripotent stem cell (hPSC) technologies enable engineered human tissue models. The organization of these multicellular constructs dictates their final form and function for research applications.
Area of Science:
- Stem cell biology
- Tissue engineering
- Developmental biology
Background:
- Human pluripotent stem cells (hPSCs) are crucial for creating in vitro tissue models.
- Advances in hPSC technology facilitate engineering complex human tissues.
- These engineered tissues serve critical roles in developmental studies, disease modeling, and drug screening.
Purpose of the Study:
- To outline the principles of in vitro human tissue construct formation using hPSCs.
- To describe the cellular organization levels in engineered tissues.
- To highlight how construct architecture influences tissue function.
Main Methods:
- Formation of multicellular hPSC constructs using scaffolds or self-assembly.
- Achieving heterotypic cell interactions via mixing or co-emergence.
- Engineering higher-order architecture through spatial constraints or cell-driven organization.
Main Results:
- Multicellular constructs are formed via scaffolds or self-assembly.
- Heterotypic interactions are established through cell mixing or co-emergence.
- Tissue architecture is controlled by external constraints or endogenous mechanisms.
Conclusions:
- The structure of hPSC-derived tissue constructs is determined by cellular organization.
- Multicellularity, heterogeneity, and organization are key factors.
- Engineered tissue form and function are dictated by construct architecture.
