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Updated: Mar 19, 2026
Author Spotlight: Utilizing Infraorbital Nerve Ligation in Mice for Investigating Trigeminal Neuropathic Pain and Treatment Strategies
Published on: March 8, 2024
Botulinum neurotoxin type-A when utilized in animals with trigeminal sensitization induced a antinociceptive effect
Elcio J Piovesan1,2, Michael Oshinsky3, Stephen Silberstein3
1Universidade Federal do Paraná, Universidade Federal do Paraná, Curitiba PR , Brasil, Universidade Federal do Paraná, Hospital das Clínicas, Serviço de Neurologia, Curitiba PR, Brasil;
Method:
Neuropathic pain was induced by surgical constriction of the infraorbital nerve in rats. A control group underwent a sham procedure consisting of surgical exposure of the nerve. Subgroups of each group received either BoNT/A or isotonic saline solution. The clinical response was assessed with the -20°C test. Animals that underwent nerve constriction developed sensitization; the sham group did not.
Results:
The sensitization was reversed by BoNT/A treatment evident 24 hours following application. Pronociceptive effect was observed in the sham group following BoNT/A.
Conclusion:
BoNT/A has an antinociceptive effect in sensitized animals and a pronociceptive effect in non-sensitized animals.
