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Related Experiment Video

Updated: Mar 17, 2026

Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue

Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue

Published on: May 17, 2021

Multiphoton Microscopy for Visualizing Lipids in Tissue.

Martin Lee¹, Alan Serrels²

¹Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, EH4 2XU, Scotland, UK. martin.lee@ed.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|July 16, 2016

Summary

This summary is machine-generated.

Coherent anti-Stokes Raman spectroscopy (CARS) enables label-free imaging of fat droplets and adipocytes. This study details a multiphoton microscope for studying labeled and unlabeled tissues, monitoring Wt1 cells in visceral fat during gestation.

Keywords:

CARS Lipids Microscopy Multiphoton Raman SHG

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Area of Science:

Biomedical Optics
Molecular Imaging
Cell Biology

Background:

Label-free imaging of adipocytes and fat droplets is crucial for understanding metabolic processes.
Coherent anti-Stokes Raman spectroscopy (CARS) offers molecular contrast based on vibrational signatures.
Multiphoton microscopy allows deep tissue imaging and can be combined with various optical contrast methods.

Purpose of the Study:

To describe the construction of a novel multiphoton microscope.
To enable the study of both fluorescently labeled and unlabeled biological tissues.
To investigate the role of Wt1 expressing cells in visceral fat depots during gestation.

Main Methods:

Construction of a multiphoton microscope system.
Utilizing coherent anti-Stokes Raman spectroscopy (CARS) for label-free imaging.
Combining CARS with other imaging modalities like second harmonic generation and two-photon fluorescence.

Main Results:

The developed multiphoton microscope successfully imaged fat droplets and adipocytes using CARS.
The system allowed for the study of both labeled and unlabeled tissue components.
The contribution of Wt1 expressing cells to visceral fat depots during gestation was monitored.

Conclusions:

Multiphoton microscopy combined with CARS is a powerful tool for label-free visualization of adipose tissue.
This technique facilitates the study of cellular contributions to tissue development and function.
The findings provide insights into the role of Wt1+ cells in adipogenesis during pregnancy.