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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Updated: Mar 16, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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The Development of Fluorescence Intensity Standards.

A K Gaigalas1, L Li1, O Henderson2

  • 1National Institute of Standards and Technology, Gaithersburg, MD 20899-8312.

Journal of Research of the National Institute of Standards and Technology
|August 9, 2016
PubMed
Summary
This summary is machine-generated.

Developing primary fluorescence intensity standards is feasible, addressing the need for comparable measurements across labs. This will advance analytical techniques by enabling reliable fluorescence intensity standard reference materials.

Keywords:
fluorescence intensityquantitative fluorescencestandards

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy

Background:

  • Fluorescence is a growing analytical technique, but its advancement is hindered by inconsistent intensity measurements across different laboratories.
  • The lack of standardized fluorescence intensity measurements limits the reliability and comparability of results in scientific assays.

Purpose of the Study:

  • To address the need for comparable fluorescence intensity measurements by developing primary fluorescence intensity standard (FIS) reference materials.
  • To facilitate the broader adoption and accuracy of fluorescence-based analytical techniques.

Main Methods:

  • The National Institute of Standards and Technology (NIST) recognizes the necessity of creating primary FIS reference materials.
  • Collaboration between federal laboratories, manufacturers, and end-users is identified as crucial for FIS development.

Main Results:

  • The development and implementation of FIS are acknowledged to present challenges.
  • The article outlines the feasibility of developing these essential fluorescence intensity standards.

Conclusions:

  • The development of primary fluorescence intensity standards is achievable and necessary.
  • Establishing FIS will overcome current limitations in fluorescence intensity measurements, promoting greater accuracy and inter-laboratory consistency.