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Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
Published on: December 23, 2022
Synthesis of 2-Thiocarbohydrates and Their Binding to Concanavalin A
Prashant Pavashe1, Elangovan Elamparuthi1, Cornelia Hettrich1
1Department of Chemistry, University of Potsdam , Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany.
Abstract:
A convenient and general synthesis of 2-thiocarbohydrates via cerium ammonium nitrate oxidation of the thiocyanate ion is described. Radical addition to glycals proceeds with excellent regio- and good stereoselectivities in only one step, deprotection affords water-soluble 2-thio saccharides. Binding studies to Con A have been performed by isothermal titration calorimetry (ITC) and saturation transfer difference (STD) NMR spectroscopy. The 2-thiomannose derivative binds even stronger to Con A than the natural substrate, offering opportunities for new lectin or enzyme inhibitors.
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