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3D printing of PLGA scaffolds for tissue engineering
Anton V Mironov1, Aleksey M Grigoryev2, Larisa I Krotova1
1Insitute of Photonic Technologies, Federal Reseach Center "Chrystallography and Photonics", Moscow, Russia.
Journal of Biomedical Materials Research. Part A
|August 21, 2016
Summary
Researchers developed a new 3D printing method for creating bioresorbable polymeric scaffolds for tissue engineering. These scaffolds showed no cytotoxicity and good cell adhesion in vitro, making them promising for regenerative medicine.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- 3D Printing Technology
Background:
- Tissue engineering requires scaffolds that support cell growth and tissue regeneration.
- Bioresorbable polymers offer advantages for temporary scaffolding in regenerative medicine.
- Precise control over scaffold architecture is crucial for optimizing cell infiltration and function.
Purpose of the Study:
- To develop a novel method for fabricating bioresorbable polymeric scaffolds with controlled architectonics for tissue engineering applications.
- To investigate the potential of extrusion 3D printing using polylactoglycolide solutions for scaffold fabrication.
- To evaluate the in vitro biocompatibility and cell interaction properties of the fabricated scaffolds.
Main Methods:
- Utilized extrusion three-dimensional (3D) printing with polylactoglycolide solutions in tetraglycol.
- Solidified printed structures in an aqueous medium to create porous matrix scaffolds.
- Characterized scaffold architecture, including pore size (0.5–500 µm) and interconnectivity, based on 3D computer models.
- Performed in vitro cytotoxicity and cell adhesion studies using NIH 3T3 fibroblasts, rabbit pancreas islet cells, and human adipose-derived stem cells.
Main Results:
- Successfully generated bioresorbable polymeric scaffolds with specified architectonics using the proposed 3D printing method.
- Fabricated matrix structures exhibited interconnected pore systems ranging from 0.5 to 500 µm.
- In vitro studies confirmed the absence of cytotoxicity for all tested cell lines.
- Demonstrated good adhesive properties of the scaffolds for mouse fibroblasts, rabbit pancreatic islets, and human mesenchymal stem cells.
Conclusions:
- The novel extrusion 3D printing method enables the fabrication of bioresorbable polymeric scaffolds with controlled architecture for tissue engineering.
- The developed scaffolds exhibit excellent biocompatibility and promote cell adhesion, indicating their potential for various regenerative medicine applications.
- This technique offers a promising approach for creating customized tissue engineering constructs with tailored properties.
