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Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging
Faris Sinjab1, Kenny Kong1, Graham Gibson2
1School of Physics and Astronomy, University Park, University of Nottingham, Nottingham, NG7 2RD, UK.
Biomedical Optics Express
|August 30, 2016
Summary
This study introduces a new multifocal Raman spectroscopy method using a digital micromirror device for faster, simultaneous spectral acquisition. This technique aids in the rapid diagnosis of skin cancer by analyzing tissue samples more efficiently.
Area of Science:
- Spectroscopy
- Biomedical Optics
- Medical Diagnostics
Background:
- Raman spectroscopy offers molecular fingerprinting for tissue analysis.
- Current methods can be time-consuming for clinical applications.
- Need for faster, adaptable spectroscopic techniques in diagnostics.
Purpose of the Study:
- To develop and validate a multifocal Raman micro-spectroscopy system.
- To enable simultaneous spectral acquisition from multiple points.
- To assess its utility in guided tumor diagnosis.
Main Methods:
- Utilized a digital micromirror device for multifocal sampling.
- Implemented real-time adaptive sampling via liquid-crystal spatial light modulator (LC-SLM).
- Combined Raman spectroscopy with confocal auto-fluorescence imaging.
Main Results:
- Demonstrated simultaneous "power-sharing" spectral acquisition from ad hoc points.
- Showcased rapid, software-controlled updating of sampling locations.
- Validated reduced measurement times for multifocal Raman and auto-fluorescence imaging.
Conclusions:
- The developed multifocal Raman system enables efficient, simultaneous spectral acquisition.
- The technique supports adaptive and selective sampling for diagnostics.
- Feasible application in guided diagnosis of human skin tumors.
