Related Experiment Video
Updated: Mar 15, 2026
14:18
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
21.9K
Multidimensional NMR approaches towards highly resolved, sensitive and high-throughput quantitative metabolomics.
Jérémy Marchand1, Estelle Martineau2, Yann Guitton3
1CEISAM, UMR6230, Université de Nantes, BP 92208, 2 rue de la Houssinière, 44322 Nantes, France; LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), 44307 Nantes, France.
Current Opinion in Biotechnology
|September 18, 2016
Summary
Two-dimensional Nuclear Magnetic Resonance (2D NMR) is becoming a powerful tool for quantitative metabolomics. Innovations in pulse sequences, analytical chemistry, and ultrafast acquisition enhance its speed and sensitivity for biological samples.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Spectroscopy
Background:
- Metabolomics involves analyzing complex biological samples.
- Multi-dimensional Nuclear Magnetic Resonance (NMR) offers potential for metabolomics.
- Current 2D NMR methods face challenges in speed and sensitivity.
Purpose of the Study:
- To highlight recent advancements in 2D NMR for quantitative metabolomics.
- To showcase innovative strategies for improving 2D NMR throughput and sensitivity.
- To discuss the potential of advanced NMR techniques in metabolomics research.
Main Methods:
- Development of innovative pulse sequences for 2D NMR.
- Integration of meticulous analytical chemistry approaches.
- Exploration of time-saving strategies like ultrafast NMR data acquisition.
- Application of hyperpolarization techniques to boost NMR sensitivity.
Main Results:
- 2D NMR is being transformed into a powerful quantitative metabolomics tool.
- Innovative methods enhance the speed and efficiency of 2D NMR analysis.
- Hyperpolarization techniques significantly increase NMR sensitivity.
Conclusions:
- Advanced 2D NMR techniques, including ultrafast acquisition and hyperpolarization, are making metabolomics more powerful and high-throughput.
- These advancements expand the application potential of NMR in biological and medical research.
- Continued innovation promises to further enhance NMR's role in complex sample analysis.
Related Concept Videos
01:15High-Resolution Mass Spectrometry (HRMS)
2.8K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.8K
01:182D NMR: Overview of Heteronuclear Correlation Techniques
866
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
866
01:12Two-Dimensional (2D) NMR: Overview
1.7K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.7K
01:162D NMR: Overview of Homonuclear Correlation Techniques
760
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
COSY90 is the standard two-dimensional (2D) COSY experiment that...
760
01:192D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
1.5K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.5K
01:21Mass Spectrometry: Complex Analysis
2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K