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11:02
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
Published on: April 29, 2011
Regulation of X-linked gene expression during early mouse development by Rlim.
Feng Wang1, JongDae Shin1,2, Jeremy M Shea3
1Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United States.
Elife
|September 20, 2016
Summary
Rlim is crucial for maintaining X chromosome inactivation in female mouse embryos, ensuring proper gene dosage. Initial Xist expression and X/A upregulation occur independently of Rlim.
Area of Science:
- Developmental Biology
- Genetics
- Epigenetics
Background:
- Mammalian X chromosome gene expression requires dosage compensation due to differing numbers of X chromosomes in males and females.
- Female mammalian embryos utilize imprinted X chromosome inactivation (iXCI) involving Rlim and Xist to regulate X gene dosage.
- Autosomal gene expression is thought to be upregulated on the single active X chromosome to balance gene dosage.
Purpose of the Study:
- To investigate the temporal regulatory functions of Rlim in imprinted X chromosome inactivation (iXCI) and Xist expression.
- To understand the role of Rlim in maintaining X-silencing and Xist RNA levels during early development.
Main Methods:
- Utilized mouse genetics and RNA sequencing (RNA-seq) on single mouse embryos.
- Analyzed the temporal dynamics of iXCI and Xist expression in relation to Rlim function.
Main Results:
- Rlim plays a critical role in maintaining high Xist RNA levels and X-silencing in female blastocyst stage embryos.
- Initial Xist expression appears to be independent of Rlim.
- X/A (X-linked/autosomal) upregulation is initiated in both male and female preimplantation embryos.
Conclusions:
- Rlim is essential for the maintenance, but not the initiation, of X chromosome inactivation in female mouse embryos.
- Early embryonic development involves coordinated regulation of X-linked and autosomal gene expression for dosage compensation.
Keywords:
Rlim/Rnf12X chromosome inactivationX upregulationXistdevelopmental biologymousemouse preimplantation developmentsingle embryo RNA-seqstem cellsMore Related Videos
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08:27A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
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