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Updated: Mar 14, 2026
09:27
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
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A functional natural deep eutectic solvent based on trehalose: Structural and physicochemical properties.
Ruipu Xin1, Suijian Qi1, Chaoxi Zeng1
1College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
Food Chemistry
|September 25, 2016
Summary
Natural deep eutectic solvents (NADESs) using trehalose and choline chloride significantly enhance protein thermostability. TCCL3-DES75 dramatically increased lysozyme stability, showing potential for protein stabilization applications.
Area of Science:
- Biochemistry
- Materials Science
- Chemical Engineering
Background:
- Protein stability is crucial for various applications.
- Natural deep eutectic solvents (NADESs) offer tunable properties.
- Trehalose and choline chloride are common components for NADESs.
Purpose of the Study:
- To prepare and characterize NADESs based on trehalose and choline chloride.
- To investigate the effect of these NADESs on protein thermostability.
- To explore the potential of NADESs as protein stabilization solvents.
Main Methods:
- Fourier transform infrared spectroscopy (FTIR) and (1)H nuclear magnetic resonance (NMR) spectroscopy for interaction analysis.
- Physicochemical property investigation across a temperature range (293.15–363.15 K).
- Differential Scanning Calorimetry (DSC) and Circular Dichroism (CD) to assess lysozyme thermostability and structural integrity.
Main Results:
- Intensive hydrogen-bonding interactions were confirmed between trehalose and choline chloride in TCCL3-DES and TCCL3-DES75.
- TCCL3-DES75 significantly enhanced the thermostability of lysozyme, indicated by DSC.
- Lysozyme in TCCL3-DES75 showed partial unfolding at 90°C and recovery at 20°C, confirmed by CD.
Conclusions:
- The NADES TCCL3-DES75 effectively stabilizes proteins like lysozyme.
- The observed stabilization is attributed to strong hydrogen-bonding interactions within the solvent.
- TCCL3-DES75 presents a promising avenue for developing novel protein stabilization strategies.
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