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Tutorial on estimating the limit of detection using LC-MS analysis, part I: Theoretical review
Hanno Evard1, Anneli Kruve1, Ivo Leito1
1University of Tartu, Institute of Chemistry, Ravila 14a, Tartu, 50411, Estonia.
Analytica Chimica Acta
|October 11, 2016
Summary
This tutorial clarifies the limit of detection (LOD) for analytical methods. Choosing the right LOD estimation depends on the method's purpose, and results vary with different approaches, requiring careful interpretation.
Area of Science:
- Analytical Chemistry
- Method Validation
Background:
- The limit of detection (LOD) is a critical validation parameter with widespread confusion due to statistical complexity.
- Existing literature offers varied perspectives, necessitating a clear guide for practitioners.
Approach:
- This two-part tutorial provides a theoretical discussion and practical examples for estimating LOD.
- It reviews LOD definitions, estimation methods, and their assumptions, comparing different approaches.
- Experimental design and factors influencing LOD, such as linearity and variability, are discussed.
Key Points:
- LOD estimation should align with the analytical method's intended use, avoiding over-effort for non-trace analysis.
- LOD estimates are sensitive to assumptions and methodology, demanding cautious application and comparison.
- Part I covers theory and definitions; Part II presents practical LC-MS/MS pesticide analysis examples.
Conclusions:
- A decision tree and detailed procedure are proposed for estimating and monitoring LOD.
- The study highlights areas needing further research and discussion in LOD estimation.
- Understanding the nuances of LOD estimation is crucial for reliable analytical method validation.
Keywords:
Decision limitDetection capabilityDetection limitLimit of detectionLiquid chromatographyMass spectrometryMore Related Videos
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