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Updated: Mar 13, 2026
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
Published on: February 25, 2017
A Quantitative Assessment of Cell-Free DNA Utilizing Several Housekeeping Genes: Measurements from Four Different
Janine Aucamp1, Abel Jacobus Bronkhorst2, Johannes F Wentzel3
1Centre for Human Metabolomics, Biochemistry Division, North-West University, Potchefstroom, 2520, South Africa. aucampj@telkomsa.net.
Abstract:
Quantitative real-time PCR (qPCR) is regularly used to quantify cell-free nucleic acids (cfNAs) in order to identify biomarkers for various pathologies. However, studies have shown notable housekeeping gene expression variation between healthy and diseased tissues and treated versus untreated cell lines. The release of housekeeping genes by four cell lines was investigated and the housekeeping gene expression between cfNAs and mRNA of the cell lines was observed in order to elucidate their relationship.
