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Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

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Detection and Quantification of KIT Mutations in ctDNA by Plasma Safe-SeqS

Johannes Fredebohm¹, Daniel H Mehnert², Ann-Kathrin Löber²

¹Sysmex Inostics GmbH, Falkenried 88, 20251, Hamburg, Germany. Fredebohm.Johannes@sysmex-inostics.com.

Advances in Experimental Medicine and Biology

|October 19, 2016

Abstract:

We have designed a highly sensitive assay based on the Safe-SeqS technology to detect de novo mutations in the KIT gene and tested its performance. This assay was applied to plasma samples of GIST patients before and after treatment with regorafenib (GRID III trial) and mutations at known and novel sites of potential secondary resistance were identified.

Keywords:

GIST KIT NGS Safe-SeqS Secondary resistance ctDNA

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