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Liquid Chromatography-Tandem Mass Spectrometry: An Emerging Technology in the Toxicology Laboratory
Yan Victoria Zhang1, Bin Wei2, Yu Zhu3
1University of Rochester Medical Center, 601 Elmwood Avenue, Box 608, Rochester, NY 14642, USA.
Abstract:
In the last decade, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has seen enormous growth in routine toxicology laboratories. LC-MS/MS offers significant advantages over other traditional testing, such as immunoassay and gas chromatography-mass spectrometry methodologies. Major strengths of LC-MS/MS include improvement in specificity, flexibility, and sample throughput when compared with other technologies. Here, the basic principles of LC-MS/MS technology are reviewed, followed by advantages and disadvantages of this technology compared with other traditional techniques. In addition, toxicology applications of LC-MS/MS for simultaneous detection of large panels of analytes are presented.
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