Real-time monitoring of vesicle pH in an endocytic pathway using an EGF-conjugated two-photon probe
Cheol Ho Heo1, Myoung Ki Cho1, Seunggun Shin2
1Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea. kimhm@ajou.ac.kr.
Chemical Communications (Cambridge, England)
|November 18, 2016
Abstract:
Herein, we developed a ratiometric two-photon probe (BHS3-EGF), derived from a pH sensitive dye and epidermal growth factor (EGF), for real-time monitoring and quantitative analysis of acidic luminal pH values during endocytic pathway activity. Two-photon microscopy imaging with BHS3-EGF allows the quantitative analysis of pH distributions of single vesicles and their dynamics in receptor-mediated endocytosis in real-time.
