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An enzymatic method for oxalate automated with the Cobas Fara centrifugal analyzer
L C Allen1, L Kadijevic, A D Romaschin
1Department of Clinical Biochemistry, Toronto General Hospital, Ontario, Canada.
Abstract:
Measurement of oxalate in urine has been automated for use with the Cobas Fara centrifugal analyzer. No sample pretreatment other than (a critical) pH adjustment is required. Between-run CVs were less than 4%. Results were linearly related to oxalate concentration to 1000 mumol/L. Ascorbic acid ingestion, up to 5 g of ascorbate daily, caused no demonstrable interference with the assay. This practical, automated method for assaying urinary oxalate is substantially faster than other chemical or enzymatic methods.
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