Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.7K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.7K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

754
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
754
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.7K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.7K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.5K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.5K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.8K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.8K
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

849
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
849

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robust single-scan ultraselective NMR.

Chemical communications (Cambridge, England)·2026
Same author

Fast and flow-compatible pseudo-3D diffusion NMR.

Chemical communications (Cambridge, England)·2026
Same author

Moving NMR infrastructures to remote access capabilities.

Progress in nuclear magnetic resonance spectroscopy·2026
Same author

Probing Hydrogen Activation in a Dimetal Dihydride Complex by Symmetric Exchange with Parahydrogen.

Journal of the American Chemical Society·2026
Same author

Impact of shared facilities in advancing solid-state NMR research: 2025 edition.

Solid state nuclear magnetic resonance·2025
Same author

Chemical hydrodynamics of nuclear spin states.

Science advances·2025
Same journal

3-Methyleneazetidine: a versatile building block for functional and post-modifiable polysulfonamides.

Chemical communications (Cambridge, England)·2026
Same journal

Synthesis of divalent galactosyl and fucosyl spiropyran derivatives for the targeted inhibition of bacterial biofilms.

Chemical communications (Cambridge, England)·2026
Same journal

Emergent cytotoxicity and mitochondrial alterations induced by a heterobimetallic Re(I)/Au(I) complex.

Chemical communications (Cambridge, England)·2026
Same journal

Cyanoacetylation of amines <i>via</i> a traceless cyanoacetyl radical: synthetic access to teriflunomide.

Chemical communications (Cambridge, England)·2026
Same journal

Loading layered double hydroxide nanoarray catalysts on a micro-curved substrate for kinetics-favorable water electrolysis reaction.

Chemical communications (Cambridge, England)·2026
Same journal

Bridging <i>in situ</i> measurements and practical conditions through gas-liquid management for CO/CO<sub>2</sub> reduction.

Chemical communications (Cambridge, England)·2026
See all related articles

Related Experiment Video

Updated: Mar 10, 2026

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging
17:06

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging

Published on: November 8, 2012

27.1K

Spatially encoded 2D and 3D diffusion-ordered NMR spectroscopy.

Ludmilla Guduff1, Ilya Kuprov2, Carine van Heijenoort1

  • 1Institut de Chimie des Substances Naturelles, CNRS UPR2301, Univ. Paris Sud, Université Paris-Saclay, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France. jeannicolas.dumez@cnrs.fr.

Chemical Communications (Cambridge, England)
|December 6, 2016
PubMed
Summary
This summary is machine-generated.

Spatial encoding significantly speeds up 3D diffusion-ordered NMR spectroscopy (SPEN DOSY) experiments. This novel approach enhances data acquisition efficiency for analyzing molecular mixtures.

More Related Videos

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

6.9K
Diffusion Imaging in the Rat Cervical Spinal Cord
10:46

Diffusion Imaging in the Rat Cervical Spinal Cord

Published on: April 7, 2015

12.3K

Related Experiment Videos

Last Updated: Mar 10, 2026

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging
17:06

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging

Published on: November 8, 2012

27.1K
In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

6.9K
Diffusion Imaging in the Rat Cervical Spinal Cord
10:46

Diffusion Imaging in the Rat Cervical Spinal Cord

Published on: April 7, 2015

12.3K

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • 3D diffusion-ordered NMR spectroscopy (DOSY) is a powerful technique for characterizing molecular mixtures.
  • Traditional 3D DOSY experiments can be time-consuming, limiting their widespread application.
  • Accelerating data acquisition is crucial for improving the efficiency of NMR-based analyses.

Purpose of the Study:

  • To introduce and evaluate a novel method for accelerating 3D DOSY experiments.
  • To demonstrate the feasibility and benefits of using spatial encoding (SPEN) in DOSY.
  • To provide a comprehensive analysis of the SPEN DOSY approach.

Main Methods:

  • Implementation of spatial encoding (SPEN) within a 3D DOSY framework.
  • Numerical simulations to analyze the SPEN DOSY methodology.
  • Experimental validation using a mixture of small molecules.

Main Results:

  • Significant acceleration in the acquisition time for 3D DOSY experiments was achieved using SPEN.
  • The SPEN DOSY approach was successfully demonstrated on a practical example.
  • Numerical analysis confirmed the effectiveness of the SPEN technique for DOSY.

Conclusions:

  • Spatial encoding (SPEN) offers a substantial advantage for accelerating 3D diffusion-ordered NMR spectroscopy (DOSY).
  • The SPEN DOSY technique enhances experimental efficiency without compromising data quality.
  • This method holds promise for broader applications in the analysis of complex molecular systems.