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Characterizing Extracellular Vesicles from Biological Fluids
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Characterizing Extracellular Vesicles from Biological Fluids

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Methods to Analyze EVs.

Bernd Giebel1, Clemens Helmbrecht2

  • 1Institute for Transfusion Medicine, University Hospital Essen, University Duisburg-Essen, Virchowstr. 179, 45147, Essen, Germany. bernd.giebel@uk-essen.de.

Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2016
PubMed
Summary
This summary is machine-generated.

Characterizing nano-sized extracellular vesicles (EVs) is difficult due to their small size. This chapter reviews current and emerging technologies for visualizing, quantifying, and characterizing these vital biological nanoparticles.

Keywords:
Dynamic light scatteringElectron microscopyExtracellular vesiclesFlow cytometryNanoparticle tracking analysisResistive pulse sensing

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Cell Biology

Background:

  • Extracellular vesicles (EVs) are nano-sized particles crucial for intercellular communication.
  • Characterizing small EVs presents significant technical challenges, limiting research.
  • Existing methods like electron microscopy have limitations for comprehensive EV analysis.

Purpose of the Study:

  • To review established and novel technologies for extracellular vesicle (EV) analysis.
  • To discuss the physical principles underlying these EV characterization methods.
  • To evaluate the advantages and limitations of various EV detection and analysis platforms.

Main Methods:

  • Review of transmission and scanning electron microscopy principles.
  • Summary of emerging technologies for nanoparticle visualization.
  • Analysis of quantification and characterization techniques for nano-sized EVs.

Main Results:

  • Established methods like electron microscopy are useful but have limitations.
  • Novel technologies are increasingly available for EV visualization and quantification.
  • A comprehensive understanding of technology principles is essential for effective EV research.

Conclusions:

  • Advancements in technology are overcoming challenges in nano-sized EV characterization.
  • Selecting appropriate technologies is critical for accurate EV research.
  • Further development of robust platforms will accelerate the study of extracellular vesicles.