Aberrant Expression Profile of Long Noncoding RNA in Human Sinonasal Squamous Cell Carcinoma by Microarray Analysis

Area of Science:

• Oncology
• Genomics
• Molecular Biology

Background:

• Sinonasal squamous cell carcinoma (SSCC) is a rare malignancy.
• The molecular mechanisms underlying SSCC development are not fully understood.
• Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.

Purpose of the Study:

• To identify aberrantly expressed lncRNAs in SSCC.
• To explore the potential functions of these lncRNAs in SSCC pathogenesis.
• To establish a foundation for understanding SSCC molecular mechanisms.

Main Methods:

• Microarray analysis of lncRNA and mRNA expression in SSCC tissues and adjacent noncancerous tissues from 6 patients.
• Gene Ontology (GO) and pathway analysis to determine gene functions.
• Quantitative real-time polymerase chain reaction (qRT-PCR) to validate key lncRNAs in 22 additional patients.

Main Results:

• Identification of 3146 differentially expressed lncRNAs and 2208 differentially expressed mRNAs in SSCC.
• GO and pathway analyses revealed SSCC-associated biological processes and cancer pathways.
• lncRNA-mRNA and gene signal networks highlighted potential key molecules in SSCC pathogenesis.
• qRT-PCR validated the differential expression of 5 selected lncRNAs.

Conclusions:

• This is the first study to screen and analyze the lncRNA expression profile in SSCC.
• The identified lncRNAs may play crucial roles in SSCC development.
• Findings provide novel insights into SSCC pathogenesis and potential diagnostic/therapeutic targets.