Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis

Area of Science:

• Environmental Science
• Marine Biology
• Analytical Chemistry

Background:

• Marine microplastic pollution is a growing global concern, with most research focusing on sea surface contamination.
• Standardized methodologies are crucial for consistent and comparable microplastic monitoring worldwide.

Purpose of the Study:

• To present a comprehensive protocol for sampling, sample preparation, separation, and chemical identification of marine microplastic particles.
• To provide a standardized method aligned with the Marine Strategy Framework Directive (MSFD) recommendations.
• To support researchers globally in their microplastic monitoring endeavors.

Main Methods:

• Sampling using a manta net deployed from a vessel.
• Separation of microplastics via visual identification and stereomicroscopy.
• Chemical identification using Attenuated Total Reflectance Fourier-Transform Infrared (ATR-FTIR) and micro FTIR spectroscopy.
• Particle size analysis using image analysis software.

Main Results:

• The protocol effectively outlines a complete workflow from sample collection to chemical identification of microplastics.
• The methodology ensures alignment with established European marine monitoring guidelines.
• The developed protocol is designed for practical application by researchers in diverse marine environments.

Conclusions:

• The presented protocol offers a robust and standardized approach to marine microplastic monitoring.
• This methodology facilitates accurate characterization of microplastic pollution, crucial for environmental assessment.
• The protocol, accompanied by a video guide, aims to enhance the consistency and comparability of microplastic research globally.