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Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy
Published on: February 5, 2017
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Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy.
Justus Hermannsdörfer1, Niels de Jonge2
1INM-Leibniz Institute for New Materials.
Journal of Visualized Experiments : Jove
|February 13, 2017
Summary
Studying nanoscale samples in liquid is now possible with Scanning Transmission Electron Microscopy (STEM) via a microfluidic chamber. This protocol details sample loading, liquid layer optimization, and dynamic process imaging for Transmission Electron Microscopy (TEM) and STEM.
Area of Science:
- Electron Microscopy
- Nanotechnology
- Materials Science
Background:
- Studying liquid-embedded samples at the nanoscale requires specialized techniques.
- Transmission Electron Microscopy (TEM) and Scanning Transmission Electron Microscopy (STEM) offer high spatial resolution.
- Integrating microfluidics with electron microscopy enables in-situ liquid sample analysis.
Purpose of the Study:
- To describe a protocol for analyzing liquid-embedded samples using STEM.
- To detail the assembly, sample loading, and data acquisition for microfluidic TEM/STEM.
- To ensure optimal liquid layer thickness and vacuum sealing for reliable nanoscale imaging.
Main Methods:
- Utilizing a microfluidic chamber with Silicon Nitride (SiN) membrane windows within the TEM/STEM specimen holder.
- Implementing specific sample loading procedures and in-situ tests for correct assembly.
- Measuring liquid thickness and acquiring images of dynamic processes.
Main Results:
- Successful demonstration of nanoscale imaging of liquid-embedded samples.
- Validation of the microfluidic system's ability to maintain a thin liquid layer and vacuum seal.
- Imaging of gold nanoparticles (AuNPs) in both pure water and saline solutions.
Conclusions:
- The described microfluidic approach is effective for nanoscale analysis of liquid samples using STEM.
- Proper assembly and liquid thickness control are critical for successful in-situ electron microscopy.
- This method allows for the study of dynamic processes in liquids at high resolution.
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