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Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone.

Mehdi Kazemzadeh-Narbat1,2, Jeroen Rouwkema1,2,3, Nasim Annabi1,2,4,5

  • 1Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.

Advanced Healthcare Materials
|February 28, 2017
PubMed
Summary
This summary is machine-generated.

This study engineered vascularized bone tissue by patterning osteogenic and angiogenic cell niches within a hydrogel. The approach successfully created mineralized bone regions surrounded by organized vasculature, improving bone formation for potential bone defect treatments.

Keywords:
bone tissue engineeringhydrogelsmicropatterningvascularization

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Vascularization is crucial for engineered bone tissue.
  • Cellular behavior and differentiation depend on their microenvironment (niche).
  • Controlling osteogenic and angiogenic niches can direct bone and vessel formation.

Purpose of the Study:

  • To engineer vascularized bone tissue using patterned osteogenic and angiogenic niches.
  • To investigate the effect of niche patterning on cellular organization and bone formation.
  • To develop a hydrogel-based construct for bone defect regeneration.

Main Methods:

  • Fabrication of photocrosslinkable hydrogel constructs using two-step photolithography.
  • Incorporation of osteogenic and angiogenic cell-laden niches.
  • Utilizing osteoinductive nanoparticles to promote osteogenesis.
  • Investigating the effect of microconstruct size on cellular function.

Main Results:

  • Simultaneous presence of osteogenic and angiogenic niches led to mineralized regions surrounded by vasculature.
  • The size of microfabricated constructs influenced cellular organization and function.
  • The angiogenic niche enhanced overall bone formation within the construct.
  • Successful engineering of vascularized bone tissue constructs.

Conclusions:

  • Patterning of osteogenic and angiogenic niches within hydrogels is an effective strategy for engineering vascularized bone tissue.
  • This approach facilitates the formation of organized vasculature and mineralized bone.
  • The developed method holds promise for treating bone defects through regenerative medicine.