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Published on: July 5, 2024
Structure-activity relationships of the ATP cofactor in ligase-catalysed oligonucleotide polymerisations
1Department of Chemistry, University of Georgia, Athens, GA 30602, USA. rhili@uga.edu.
Abstract:
A T4 DNA ligase-catalysed oligonucleotide polymerisation process has been recently developed to enable the incorporation of multiple functional groups throughout a nucleic acid polymer. T4 DNA ligase requires ATP as a cofactor to catalyse phosphodiester bond formation during the polymerisation process. Herein, we describe the structure-activity relationship of ATP within the context of T4 DNA ligase-catalyzed oligonucleotide polymerisation. Using high-throughput sequencing, we study not only the influence of ATP modification on polymerisation efficiency, but also on the fidelity and sequence bias of the polymerisation process.
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