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En face coherence microscopy [Invited].

Olivier Thouvenin1, Kate Grieve2, Peng Xiao1

  • 1Institut Langevin ESPCI, PSL Research University, CNRS UMR7587 1rue Jussieu, Paris F75005, France.

Biomedical Optics Express
|March 9, 2017
PubMed
Summary
This summary is machine-generated.

En face coherence microscopy, also known as flying spot or full field optical coherence tomography (FF-OCT/FF-OCM), offers unique advantages in biological imaging. This review explores its applications, contrasting it with B-scan tomography for enhanced resolution and contrast.

Keywords:
(120.5820) Scattering measurements(170.1650) Coherence imaging(170.4580) Optical diagnostics for medicine(180.1655) Coherence tomography(180.3170) Interference microscopy

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Area of Science:

  • Biomedical Optics
  • Microscopy
  • Optical Coherence Tomography

Background:

  • En face coherence microscopy, a variant of optical coherence tomography (OCT), utilizes source coherence length for sectioning.
  • This technique, encompassing flying spot and full field optical coherence tomography (FF-OCT/FF-OCM), provides en face imaging capabilities.

Purpose of the Study:

  • To analyze the advantages and disadvantages of en face coherence microscopy compared to B-scan tomography.
  • To evaluate different illumination and imaging strategies, including coherent vs. incoherent illumination and scanning vs. full field approaches.
  • To showcase diverse applications of en face coherence microscopy, highlighting the value of endogenous and exogenous contrasts.

Main Methods:

  • Comparative analysis of en face and B-scan tomography regarding resolution.
  • Investigation of coherent versus incoherent illumination and the impact of aberrations.
  • Evaluation of scanning versus full field imaging techniques.

Main Results:

  • En face coherence microscopy offers distinct benefits and drawbacks compared to B-scan tomography.
  • Illumination strategies and aberration influence impact image quality.
  • Full field imaging presents an alternative to scanning methods.

Conclusions:

  • En face coherence microscopy, particularly FF-OCT/FF-OCM, demonstrates significant potential in various imaging applications.
  • The integration of endogenous and exogenous contrasts enhances morphological information.
  • Future developments are promising in several key application domains for en face coherence microscopy.