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Updated: Mar 6, 2026

Single-cell Microinjection for Cell Communication Analysis

Single-cell Microinjection for Cell Communication Analysis

Published on: February 26, 2017

Single-cell Microinjection for Cell Communication Analysis.

Anael Viana Pinto Alberto¹, André G Bonavita¹, Antonio A Fidalgo-Neto¹

¹Institute Oswaldo Cruz, Laboratory of Cellular Communication, Oswaldo Cruz Foundation.

Journal of Visualized Experiments : Jove

|March 14, 2017

Summary

This summary is machine-generated.

This study demonstrates a microinjection technique to visualize cell communication through gap junctions. Lucifer Yellow dye diffusion in living cells confirms functional gap junction pathways in real-time.

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Area of Science:

Cell Biology
Biophysics

Background:

Gap junctions are crucial for intercellular communication, formed by connexins mediating the exchange of ions and small molecules.
These junctions play vital roles in physiological processes including neural signaling and cardiac function.

Purpose of the Study:

To detail a single-cell microinjection method using Lucifer Yellow to visualize gap junction communication in living cells.
To establish a real-time assessment of dye diffusion for evaluating gap junction functionality.

Main Methods:

Preparation of cells and micropipettes for microinjection.
Utilizing a micromanipulator for precise injection of low molecular weight fluorescent dye into epithelial cells.

Main Results:

Successful visualization of intercellular communication via gap junctions through dye transfer.
Real-time evaluation of dye diffusion patterns indicating functional gap junction connectivity.

Conclusions:

Single-cell microinjection of Lucifer Yellow is an effective technique for studying gap junction function.
This method allows for direct, real-time assessment of cellular communication pathways mediated by gap junctions.