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Related Concept Videos

Cooperative Allosteric Transitions01:58

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Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
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Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
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The Equilibrium Binding Constant and Binding Strength02:18

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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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Assessing cooperativity in supramolecular systems.

Larissa K S von Krbek1, Christoph A Schalley, Pall Thordarson

  • 1Institut für Chemie und Biochemie, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany. christoph@schalley-lab.de.

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|March 30, 2017
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Summary
This summary is machine-generated.

This review categorizes cooperativity in supramolecular complexes, introducing methods to quantify it using thermodynamic cooperativity factors. It highlights isothermal titration calorimetry (ITC) as a key technique for analyzing these interactions.

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Area of Science:

  • Supramolecular Chemistry
  • Chemical Thermodynamics

Background:

  • Cooperativity is crucial for the function of many supramolecular complexes.
  • Understanding and quantifying cooperativity is essential for designing novel molecular systems.

Purpose of the Study:

  • To systematically categorize different types of cooperativity in supramolecular complexes.
  • To discuss thermodynamic approaches for quantifying cooperativity using cooperativity factors.
  • To review methods for determining thermodynamic data, emphasizing isothermal titration calorimetry (ITC).

Main Methods:

  • Systematic categorization of cooperativity (aggregation, intermolecular, intramolecular, interannular).
  • Thermodynamic analysis using cooperativity factors.
  • Overview of experimental techniques for thermodynamic data acquisition, focusing on ITC.

Main Results:

  • A proposed classification of cooperativity into four distinct types.
  • Demonstration of thermodynamic quantification methods for cooperativity.
  • Highlighting the advantages of ITC in supramolecular chemistry research.

Conclusions:

  • A structured framework for understanding cooperativity in supramolecular chemistry is presented.
  • Thermodynamic analysis provides valuable insights into complex molecular interactions.
  • ITC is a powerful, underutilized tool for studying supramolecular systems.