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High Density Individually Addressable Nanowire Arrays Record Intracellular Activity from Primary Rodent and Human
Ren Liu, Renjie Chen, Ahmed T Elthakeb
1Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute , 10901 North Torrey Pines Road, La Jolla, California 92037, United States.
Nano Letters
|April 7, 2017
Summary
Researchers developed a scalable nanowire-on-lead platform for high-resolution neural recordings. This new method enables sensitive electrophysiological measurements from human induced pluripotent stem cell-derived neurons for disease modeling.
Area of Science:
- Neuroscience
- Bioengineering
- Materials Science
Background:
- Electrophysiological recordings are crucial for understanding neuronal function and disease.
- Existing methods face limitations in scalability, spatial resolution, and long-term monitoring.
- Human induced pluripotent stem cell (hiPSC)-derived neurons offer a powerful model for studying neurological disorders.
Purpose of the Study:
- To introduce a novel nanowire-on-lead integration scheme for advanced electrophysiological recordings.
- To demonstrate the scalability and high spatial resolution of the developed nanowire arrays.
- To assess the platform's capability for recording from primary neurons and hiPSC-derived neurons.
Main Methods:
- Fabrication of vertical nanowire arrays using a hybrid integration scheme with submicrometer spacing.
- Integration of nanowire arrays with standard semiconductor fabrication processes.
- Electrophysiological recordings from mouse/rat primary neurons and hiPSC-derived neurons at various in vitro days.
Main Results:
- Achieved independent electrical addressability and superior spatial resolution with the nanowire-on-lead approach.
- Demonstrated scalable fabrication compatible with integrated circuit technologies.
- Obtained high signal-to-noise ratios and detected subthreshold postsynaptic potentials (PSPs) in neuronal recordings.
- Recorded electrical activity from rodent neurons (8-14 DIV) and hiPSC-derived neurons (6 weeks in vitro) with signal amplitudes up to 99 mV.
Conclusions:
- The nanowire-on-lead platform enables longitudinal electrophysiological studies of synaptic activity.
- This technology is critical for advancing research in human iPSC-based neuronal network disease models.
- Facilitates understanding of neurological disease mechanisms and accelerates drug development for neurological disorders.