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Compact interferometric module for full-field interferometric phase microscopy with low spatial coherence

Amit Nativ, Natan T Shaked

    Optics Letters
    |April 15, 2017
    PubMed
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    We developed a new off-axis interferometric module for quantitative phase imaging. This compact device works with low spatial coherence illumination, enabling stable, low-noise imaging of reflective samples.

    Area of Science:

    • Optical imaging
    • Microscopy
    • Interferometry

    Background:

    • Quantitative phase imaging (QPI) provides label-free contrast for transparent and reflective samples.
    • Traditional interferometers often require highly coherent light sources and complex alignment procedures.
    • Integrating QPI with existing microscopy setups using low spatial coherence illumination remains a challenge.

    Purpose of the Study:

    • To develop a compact, external off-axis interferometric module for quantitative phase imaging.
    • To achieve stable interference with low spatial coherence illumination across the entire field of view.
    • To enable low-noise QPI compatible with standard microscopes.

    Main Methods:

    • Design and construction of a compact, external off-axis interferometric module.

    Related Experiment Videos

  • Utilizing a low spatial coherence light source for illumination.
  • Demonstration of interference and imaging properties of the module.
  • Application of the module for quantitative phase imaging of reflective samples.

    • Main Results:

    • The proposed interferometric module successfully achieved interference with low spatial coherence illumination over the entire field of view.
    • The interferometer demonstrated ease of alignment and high stability.
    • Quantitative phase imaging of reflective samples was performed with a low degree of spatial noise.

    Conclusions:

    • The developed off-axis interferometric module is a versatile and practical tool for quantitative phase imaging.
    • It allows for low-noise QPI using readily available low spatial coherence light sources and existing microscopes.
    • This technology facilitates advanced imaging of reflective samples without complex setups.