[Radioreceptor method of determination of opioid peptides in the rat brain]

E G Davletov, I N Kurochkin, S V Zaĭtsev

Voprosy Meditsinskoi Khimii

|July 1, 1988

Abstract:

A procedure is described for quantitative radioreceptor estimation of opioid peptides in rat brain using lyophilized membrane preparations. As opiate receptors exhibit high rate of heterogeneity estimation of delta- and mu-opioid activities was carried out by means of displacing of 3H-DADLE and 3H-DAGO, respectively. Content of opioid peptides in brain of preadolescent rats was studied during experimental burn trauma using the procedure developed. An increase in the delta- and mu-opioid activities was found in rat brain tissue within one day after thermal burns.

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Abstract:

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